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Image Search Results
Journal: Acta Naturae
Article Title: An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity
doi: 10.32607/actanaturae.11776
Figure Lengend Snippet: Frequency of seropositive serum samples in the analyzed groups. ( A ) Frequency of anti-RBD IgG positive serum samples per group. ( B ) Frequency of RBD-nAb seropositive serum samples among RBD-IgG-positive samples. ( C ) Frequencies of occurrence of RBD-nAb-positive samples in the groups. Statistical significance of the intergroup differences was determined using the Fisher’s exact test (* p < 0.05; **** p < 0.0001)
Article Snippet: The subclasses of RBD-specific IgG were analyzed according to the protocol described above, even though the calibration curves were not plotted, and the horseradish peroxidase conjugates of the following antibodies were used: anti-human IgG1 antibodies (HyTest, Finland, Cat. # 1G2cc), anti-human IgG2 antibodies (HyTest, Finland, Cat. # 1G5),
Techniques:
Journal: Acta Naturae
Article Title: An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity
doi: 10.32607/actanaturae.11776
Figure Lengend Snippet: Concentration of SARS-CoV-2 RBD-specific IgG and the activity of RBD-ACE2 neutralizing antibodies in seropositive serum samples measured by sVNT. ( A ) Concentration of SARS-CoV-2 RBD-specific IgG in seropositive serum samples. ( B ) Neutralizing activity of RBD-specific antibodies (RBD-nAb) in seropositive serum samples. The statistical significance of intergroup differences was determined using the Kruskal–Wallis test (* p < 0.05; ** p < 0.01; **** p < 0.0001)
Article Snippet: The subclasses of RBD-specific IgG were analyzed according to the protocol described above, even though the calibration curves were not plotted, and the horseradish peroxidase conjugates of the following antibodies were used: anti-human IgG1 antibodies (HyTest, Finland, Cat. # 1G2cc), anti-human IgG2 antibodies (HyTest, Finland, Cat. # 1G5),
Techniques: Concentration Assay, Activity Assay
Journal: Acta Naturae
Article Title: An ELISA Platform for the Quantitative Analysis of SARS-CoV-2 RBD-neutralizing Antibodies As an Alternative to Monitoring of the Virus-Neutralizing Activity
doi: 10.32607/actanaturae.11776
Figure Lengend Snippet: Linear regression analysis of the serum antibody RBD-ACE2 neutralizing activity and RBD-specific IgG concentration. Double seropositive (RBD-IgG+ and RBD-nAb+) serum samples are shown as white circles; negative samples are shown as gray circles. The 95% confidence intervals and activity and concentration thresholds are shown with dotted lines. R is the Pearson correlation coefficient; K is the slope of the regression line; CI is the 95% confidence interval
Article Snippet: The subclasses of RBD-specific IgG were analyzed according to the protocol described above, even though the calibration curves were not plotted, and the horseradish peroxidase conjugates of the following antibodies were used: anti-human IgG1 antibodies (HyTest, Finland, Cat. # 1G2cc), anti-human IgG2 antibodies (HyTest, Finland, Cat. # 1G5),
Techniques: Activity Assay, Concentration Assay